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sars-cov-2 (2019-ncov) spike gene orf cdna clone expression plasmid, n-his tag  (Sino Biological)


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    Sino Biological sars-cov-2 (2019-ncov) spike gene orf cdna clone expression plasmid, n-his tag
    Sars Cov 2 (2019 Ncov) Spike Gene Orf Cdna Clone Expression Plasmid, N His Tag, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 130 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/full+length+wuhan+wt+spike/SARS-CoV-2+(2019-nCoV)+Spike+Gene+ORF+cDNA+clone+expression+plasmid%2C+N-His+tag/custom%40vg40589-nh%4010%2E1016%2Fj%2Ecolsurfa%2E2024%2E135828
    Average 94 stars, based on 130 article reviews
    sars-cov-2 (2019-ncov) spike gene orf cdna clone expression plasmid, n-his tag - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Transfection:

    Article Title: Multivalent IgM scaffold enhances the therapeutic potential of variant-agnostic ACE2 decoys against SARS-CoV-2
    Article Snippet: The binding kinetics were analyzed using the 1:1 binding model and kinetic analyses were performed using Biacore T200 Evaluation Software v3.0. .. HEK293-6E cells were transiently transfected with full-length Wuhan (WT) spike (pCMV3 backbone, Sino Biological, #VG40589-UT) or mock GFP negative control (pD2610-CMV(v23)-GFP). .. Spike protein expression on transfected HEK293-6E cells was confirmed by flow cytometry using QuantumTM Simply Cellular® anti-Human IgG beads (Bangs Laboratories, #816) and 15 μg/mL anti-spike-AF647 (eBioscience, #51-6490-82) antibody according to the manufacturer’s protocol.

    Negative Control:

    Article Title: Multivalent IgM scaffold enhances the therapeutic potential of variant-agnostic ACE2 decoys against SARS-CoV-2
    Article Snippet: The binding kinetics were analyzed using the 1:1 binding model and kinetic analyses were performed using Biacore T200 Evaluation Software v3.0. .. HEK293-6E cells were transiently transfected with full-length Wuhan (WT) spike (pCMV3 backbone, Sino Biological, #VG40589-UT) or mock GFP negative control (pD2610-CMV(v23)-GFP). .. Spike protein expression on transfected HEK293-6E cells was confirmed by flow cytometry using QuantumTM Simply Cellular® anti-Human IgG beads (Bangs Laboratories, #816) and 15 μg/mL anti-spike-AF647 (eBioscience, #51-6490-82) antibody according to the manufacturer’s protocol.



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    Sino Biological sars-cov-2 (2019-ncov) spike gene orf cdna clone expression plasmid, n-his tag
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    Dot plot with means and 95% confidence intervals (CI) of antigen-specific outcomes (MFI—BSA) for serum IgG, covering SARS-CoV-2 (including variants) and human endemic coronaviruses (OC43, HKU1, NL63, 229E) with log10 y-axis. See for plots with linear y-axis. MFI, median fluorescence intensity. N, Nucleocapsid. S, Spike. RBD, Receptor Binding Protein. hCoV, human endemic coronaviruses. WT, <t>wild-type</t> <t>(Wuhan).</t>
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    Binding kinetics of ACE2 decoy variants and clinical comparators to recombinant spike protein RBD as measured by SPR.
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    Image Search Results


    Dot plot with means and 95% confidence intervals (CI) of antigen-specific outcomes (MFI—BSA) for serum IgG, covering SARS-CoV-2 (including variants) and human endemic coronaviruses (OC43, HKU1, NL63, 229E) with log10 y-axis. See for plots with linear y-axis. MFI, median fluorescence intensity. N, Nucleocapsid. S, Spike. RBD, Receptor Binding Protein. hCoV, human endemic coronaviruses. WT, wild-type (Wuhan).

    Journal: PLOS ONE

    Article Title: Minding the margins: Evaluating the impact of COVID-19 among Latinx and Black communities with optimal qualitative serological assessment tools

    doi: 10.1371/journal.pone.0307568

    Figure Lengend Snippet: Dot plot with means and 95% confidence intervals (CI) of antigen-specific outcomes (MFI—BSA) for serum IgG, covering SARS-CoV-2 (including variants) and human endemic coronaviruses (OC43, HKU1, NL63, 229E) with log10 y-axis. See for plots with linear y-axis. MFI, median fluorescence intensity. N, Nucleocapsid. S, Spike. RBD, Receptor Binding Protein. hCoV, human endemic coronaviruses. WT, wild-type (Wuhan).

    Article Snippet: The following SARS-CoV-2 antigens were coupled to Luminex MagPlex Microspheres as indicated by the manufacturer: Wild-type (WT; Wuhan) full-length spike (S), WT nucleocapsid (N), WT receptor-binding domain (RBD), RBD alpha, RBD beta, RBD gamma, RBD delta, RBD lambda, and RBD omicron.

    Techniques: Fluorescence, Binding Assay

    Binding kinetics of ACE2 decoy variants and clinical comparators to recombinant spike protein RBD as measured by SPR.

    Journal: mAbs

    Article Title: Multivalent IgM scaffold enhances the therapeutic potential of variant-agnostic ACE2 decoys against SARS-CoV-2

    doi: 10.1080/19420862.2023.2212415

    Figure Lengend Snippet: Binding kinetics of ACE2 decoy variants and clinical comparators to recombinant spike protein RBD as measured by SPR.

    Article Snippet: HEK293-6E cells were transiently transfected with full-length Wuhan (WT) spike (pCMV3 backbone, Sino Biological, #VG40589-UT) or mock GFP negative control (pD2610-CMV(v23)-GFP).

    Techniques: Binding Assay, Recombinant